Advances in Mushroom Cultivation Technologies: Substrate Optimization, Controlled Environment and Strategies for Yield Enhancement
Keywords:
Mushroom cultivation, Substrate optimization, Controlled-environment systems, Yield enhancement, Sustainable agricultureAbstract
Mushroom farming serves sustainable farming practices because it produces healthy food while it transforms farm waste into useful organic matter. Research studies during the last few years have concentrated on developing better substrate materials and environmental control systems and yield optimization methods to address the current restrictions of conventional cultivation methods. The review evaluates modern mushroom cultivation methods through an analysis of substrate enhancement techniques and controlled growing systems and complete yield enhancement methods. The research team used Web of Science and Scopus and Google Scholar to find relevant peer-reviewed studies which appeared during the last five years. The research findings demonstrate that optimized ligno-cellulosic substrates made from agro-industrial waste products will produce better mycelial growth and biological efficiency when researchers use suitable pre-treatment methods and add necessary nutrients. The implementation of sensor-based monitoring systems and IoT-enabled automation and IoT systems enables farmers to maintain exact control over temperature and humidity and CO₂ levels and light exposure which leads to better crop stability and enhanced product quality and decreased chances of contamination. The most effective yield enhancement results from an integrated system which selects genetic strains and implements optimized agricultural practices and stress management and bio-stimulant treatment according to substrate composition and environmental conditions. The upcoming developments in artificial intelligence-based monitoring systems and sustainable substrate technologies and smart farming methods will enhance both production levels and resource utilization efficiency. The review shows how integrated cultivation systems support sustainable mushroom farming which protects food availability and promotes circular agricultural practices.
References
Agba, M. O., Markson, A. A., Oni, J. O., & Bassey, G. A. (2021). Growth and yield impact of oyster mushroom Pleurotus ostreatus (Jacq.) P. Kumm. cultivated on different agricultural wastes. Journal of Bioscience and Agriculture Research, 27(1), 2225–2233. https://doi.org/10.18801/jbar.270121.271
Argaw, B., Tesfay, T., Godifey, T., & Asres, N. (2023). Growth and yield performance of oyster mushroom (Pleurotus ostreatus (Jacq.: Fr.) Kummer) using waste leaves and sawdust. International Journal of Agronomy, 2023, Article 8013491. https://doi.org/10.1155/2023/8013491
Badoni, P., & Siddiqui, S. A. (2025). Metamorphosis of mushroom production from tradition to automation. Discover Applied Sciences, 7, Article 974. https://doi.org/10.1007/s44172-025-00974-3
Barauskas, R., Kriščiūnas, A., Čalnerytė, D., Pilipavičius, P., Fyleris, T., Daniulaitis, V., & Mikalauskis, R. (2022). Approach of AI-based automatic climate control in white button mushroom growing hall. Agriculture, 12(11), Article 1921. https://doi.org/10.3390/agriculture12111921
Barua, B. S., Nigaki, A., & Kataoka, R. (2024). A new recycling method through mushroom cultivation using food waste: Optimization of mushroom bed medium using food waste and agricultural use of spent mushroom substrates. Recycling, 9(4), Article 58. https://doi.org/10.3390/recycling9040058
Carrasco, J., Zied, D. C., Navarro, M. J., Gea, F. J., & Pardo-Giménez, A. (2021). Commercial cultivation techniques of mushrooms. In D. C. Zied & A. Pardo-Giménez (Eds.), Advances in macrofungi (pp. 11–40). CRC Press. https://doi.org/10.1201/9781003031687-2
Chahal, S., Sindhu, A., Singh, A., & Sindhu, S. C. (2024). Exploring extraction techniques for medicinal mushroom bioactive compounds: A comprehensive review of advantages and limitations. The Pharma Innovation Journal, 13(2), 272–280.
Chen, L., Qian, L., Zhang, X., Li, J., Zhang, Z., & Chen, X. (2022). Research progress on indoor environment of mushroom factory. International Journal of Agricultural and Biological Engineering, 15(1), 25–32. https://doi.org/10.25165/j.ijabe.20221501.6985
Desisa, B., Muleta, D., Dejene, T., Jida, M., Goshu, A., & Martín-Pinto, P. (2023). Substrate optimization for shiitake (Lentinula edodes (Berk.) Pegler) mushroom production in Ethiopia. Journal of Fungi, 9(8), Article 811. https://doi.org/10.3390/jof9080811
Dey, B., Ador, M. A. H., Haque, M. M. U., Ferdous, J., Halim, M. A., Uddin, M. B., & Ahmed, R. (2024). Strategic insights for sustainable growth of mushroom farming industry in Bangladesh. Heliyon, 10(17), Article e33578. https://doi.org/10.1016/j.heliyon.2024.e33578
Dou, T., Zhang, K., Shi, X., Liu, W., Yu, F., & Liu, D. (2025). Crop–mushroom rotation: A comprehensive review of its multifaceted impacts on soil quality, agricultural sustainability, and ecosystem health. Agronomy, 15(3), Article 563. https://doi.org/10.3390/agronomy15030563
Fadugba, S. E., Jeeva, N., Fadugba, A. E., & Faweya, O. (2025). Mathematical modeling for the analysis and optimization of nutrient dynamics in edible mushrooms. European Journal of Pure and Applied Mathematics, 18(4), 7190–7199.
Gao, Y., Wu, Z., Li, W., Sun, H., Chai, Y., Li, T., & Qin, P. (2023). Expanding the valorization of waste mushroom substrates in agricultural production. Environmental Science and Pollution Research, 30(2), 2355–2373. https://doi.org/10.1007/s11356-022-22853-6
Gundoshmian, T. M., Ardabili, S., Csaba, M., & Mosavi, A. (2022). Modeling and optimization of oyster mushroom growth using artificial neural networks. Mathematical Biosciences and Engineering, 19(10), 9749–9768. https://doi.org/10.3934/mbe.2022454
Hirai, M. M. (2025). Cultivo de cogumelo Pleurotus ostreatus em resíduos da cultura de urucum (Master’s thesis). Universidade Federal do Espírito Santo, Brazil.
Hu, W., Gou, L., Hu, L., Wang, S., Liang, T., & Zhou, N. (2025). Effect of acid modification of biochar derived from spent mushroom substrate on oyster mushroom production. Scientific Reports, 15, Article 30955. https://doi.org/10.1038/s41598-025-30955-4
Hu, Y., Mortimer, P. E., Hyde, K. D., Kakumyan, P., & Thongklang, N. (2021). Mushroom cultivation for soil amendment and bioremediation. Circular Agricultural Systems, 1(1), 1–14. https://doi.org/10.1017/S2633903X21000042
Irwanto, F., Hasan, U., Lays, E. S., De La Croix, N. J., Mukanyiligira, D., Sibomana, L., & Ahmad, T. (2024). IoT and fuzzy logic integration for improved substrate environment management in mushroom cultivation. Smart Agricultural Technology, 7, Article 100427. https://doi.org/10.1016/j.atech.2023.100427
Itrat, N., Hasanath, S. A. F., & Ali, A. (2025). Mushrooms as natural antioxidants. In Mushroom bioactives: Bridging food, biotechnology, and nanotechnology for health and innovation (pp. 45–67). Elsevier.
Jarial, R. S., Jarial, K., & Bhatia, J. N. (2024). Comprehensive review on oyster mushroom species. Heliyon, 10(5), Article e27149. https://doi.org/10.1016/j.heliyon.2024.e27149
Kavaliauskas, Ž., Šajev, I., Gecevičius, G., & Čapas, V. (2022). Intelligent control of mushroom growing conditions. Applied Sciences, 12(24), Article 13040. https://doi.org/10.3390/app122413040
Nakazawa, T., Kawauchi, M., Otsuka, Y., Han, J., Koshi, D., Schiphof, K., & Honda, Y. (2024). Pleurotus ostreatus as a model mushroom. Applied Microbiology and Biotechnology, 108(1), 217–232. https://doi.org/10.1007/s00253-023-12866-7
Ravindran, S., Ramlan, N. H., Shah, A. S. M., Yudin, A. S. M., Rahman, R. A., & Faudzi, A. A. M. (2025). Enhancing mushroom cultivation through IoT-based monitoring. In Proceedings of the IEEE 8th International Conference on Electrical, Control and Computer Engineering (pp. 326–331). IEEE. https://doi.org/10.1109/InECCE60024.2025.10512345
Suwannarach, N., Kumla, J., Zhao, Y., & Kakumyan, P. (2022). Impact of cultivation substrate and microbial community on improving mushroom productivity. Biology, 11(4), Article 569. https://doi.org/10.3390/biology11040569
Zou, G., Li, T., Mijakovic, I., & Wei, Y. (2024). Synthetic biology enables mushrooms to meet emerging sustainable challenges. Frontiers in Microbiology, 15, Article 1337398. https://doi.org/10.3389/fmicb.2024.133739
